• Corpus ID: 113401935

The ubiquitin – proteasome system plays essential roles in presenting an 8-mer CTL epitope expressed in APC to corresponding CD 8 1 T cells

@inproceedings{Duan2006TheU,
  title={The ubiquitin – proteasome system plays essential roles in presenting an 8-mer CTL epitope expressed in APC to corresponding CD 8 1 T cells},
  author={Xuefeng Duan and Hajime Hisaeda and Jianying Shen and Liping Tu and Takashi Imai and Bin Chou and Shigeo Murata and Tomoki Chiba and Keiji Tanaka and Hans J{\"o}rg Fehling and Takaomi Koga and Katsuo Sueishi and Kunisuke Himeno},
  year={2006}
}
MUT1 is an H-2K-restricted 8-mer CTL epitope expressed in Lewis lung carcinoma (3LL) tumor cells derived from C57BL/6 (B6) mice. We constructed a chimeric gene encoding ubiquitin-fused MUT1 (pUB-MUT1). By using a gene gun, B6 mice were immunized with the gene prior to challenge with 3LL tumor cells. Tumor growth and lung metastasis were prominently suppressed in mice immunized with pUB-MUT1 but only slightly in those immunized with the MUT1 gene (pMUT) alone. CD8 T cells were confirmed to be… 

Figures from this paper

References

SHOWING 1-10 OF 64 REFERENCES
A role for the proteasome regulator PA28alpha in antigen presentation.
TLDR
Enhanced expression of PA28alpha at a level similar to that obtained after IFN-gamma induction resulted in a marked enhancement of recognition by pp89-specific cytotoxic T cells; the presentation of influenza nucleoprotein was also significantly improved.
A novel DNA vaccine based on ubiquitin–proteasome pathway targeting ‘self’-antigens expressed in melanoma/melanocyte
TLDR
These findings provide evidence for the first time that naked DNA vaccines encoding a ubiquitin-fused self-antigen preferentially induce the main effector CD8+ T cells through efficient proteolysis mediated by the ubiquitIn–proteasome pathway, and lead the way to strategies aimed at targeting tissue differentiation antigens expressed by tumors.
Ubiquitin‐fusion degradation pathway plays an indispensable role in naked DNA vaccination with a chimeric gene encoding a syngeneic cytotoxic T lymphocyte epitope of melanocyte and green fluorescent protein
TLDR
Taken together, GFP‐TRP‐2 processed by cytosolic proteasome played a central role in breaking peripheral tolerance to a melanoma/melanocyte antigen, TRp‐2181−188, by activating CD8+ CTL specific for TRP‐ 2181‐188.
The role of the ubiquitin-proteasome pathway in MHC class I antigen processing: implications for vaccine design.
TLDR
The different ways by which tumors and viruses have been found to target the proteasome system to avoid MHC class I presentation of their antigens are reviewed, and recent progressions in the development of computer assisted approaches to predict CTL epitopes within larger protein sequences are discussed.
IL-12 plasmid-enhanced DNA vaccination against carcinoembryonic antigen (CEA) studied in immune-gene knockout mice
TLDR
It is concluded that the immune requirements for tumor rejection are stringent, involving multiple mechanisms which are all enhanced by IL-12.
The proteasome activator 11 S REG (PA28) and class I antigen presentation.
TLDR
The present review focuses on the structural properties of REG molecules and on the evidence that REGalpha/beta functions in the Class I immune response.
Regulation of T-helper-1 versus T-helper-2 activity and enhancement of tumor immunity by combined DNA-based vaccination and nonviral cytokine gene transfer
TLDR
It is shown that immunity elicited by DNA vaccination against CEA can be biased to a protective type ( high Th1 and CTL activity) or nonprotective type (high Th2 and low CTLActivity) by i.m. coinjection of cytokine-expressing plasmids.
An autologous oral DNA vaccine protects against murine melanoma.
  • R. Xiang, H. Lode, R. Reisfeld
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 2000
We demonstrated that peripheral T cell tolerance toward murine melanoma self-antigens gp100 and TRP-2 can be broken by an autologous oral DNA vaccine containing the murine ubiquitin gene fused to
Identification of shared tumor-associated antigen peptides between two spontaneous lung carcinomas.
TLDR
Using CTL cross-reaction assays, peptide extraction, HPLC fractionation, and reverse transcriptase-PCR amplification, it is shown that clones of another spontaneous C57BL/6 lung carcinoma, CMT 64, share TAA peptides with the 3LL carcinoma.
...
1
2
3
4
5
...