• Corpus ID: 113401935

The ubiquitin – proteasome system plays essential roles in presenting an 8-mer CTL epitope expressed in APC to corresponding CD 8 1 T cells

  title={The ubiquitin – proteasome system plays essential roles in presenting an 8-mer CTL epitope expressed in APC to corresponding CD 8 1 T cells},
  author={Xuefeng Duan and Hajime Hisaeda and Jianying Shen and Liping Tu and Takashi Imai and Bin Chou and Shigeo Murata and Tomoki Chiba and Keiji Tanaka and Hans J{\"o}rg Fehling and Takaomi Koga and Katsuo Sueishi and Kunisuke Himeno},
MUT1 is an H-2K-restricted 8-mer CTL epitope expressed in Lewis lung carcinoma (3LL) tumor cells derived from C57BL/6 (B6) mice. We constructed a chimeric gene encoding ubiquitin-fused MUT1 (pUB-MUT1). By using a gene gun, B6 mice were immunized with the gene prior to challenge with 3LL tumor cells. Tumor growth and lung metastasis were prominently suppressed in mice immunized with pUB-MUT1 but only slightly in those immunized with the MUT1 gene (pMUT) alone. CD8 T cells were confirmed to be… 

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    Proceedings of the National Academy of Sciences of the United States of America
  • 2000
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