The two-step electrochemical oxidation of alcohols using a novel recombinant PQQ alcohol dehydrogenase as a catalyst for a bioanode.

@article{Takeda2013TheTE,
  title={The two-step electrochemical oxidation of alcohols using a novel recombinant PQQ alcohol dehydrogenase as a catalyst for a bioanode.},
  author={Kouta Takeda and Hirotoshi Matsumura and Takuya Ishida and Masahiro Samejima and Kiyohiko Igarashi and Nobuhumi Nakamura and Hiroyuki Ohno},
  journal={Bioelectrochemistry},
  year={2013},
  volume={94},
  pages={
          75-8
        }
}
A bioanode has been developed based on the oxidation of ethanol by the recombinant pyrroloquinoline quinone (PQQ) dependent alcohol dehydrogenase from Pseudomonas putidaKT2440 heterologously expressed in Pichia pastoris. The apo form of the recombinant protein (PpADH) was purified and displayed catalytic activity for binding PQQ in the presence of Ca(2+). PpADH exhibited broad substrate specificity towards various alcohols and aldehydes. The Km values for the aldehydes of PpADH were increased… CONTINUE READING
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