The tumor suppressor p53 is subject to both nuclear import and export, and both are fast, energy-dependent and lectin-inhibited

Abstract

Human p53 was expressed in E. coli, purified, labeled with fluorescein iodoacetamide (IAF) and characterized for sequence-specific DNA binding and epitope disposition. Injected into the cytoplasm or nuclei of 3T3 cells IAF-p53 was imported into or exported from nuclei within minutes. Import was inhibited by coinjection of the lectin wheat germ agglutinine… (More)
DOI: 10.1038/sj.onc.1200949

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@article{Middeler1997TheTS, title={The tumor suppressor p53 is subject to both nuclear import and export, and both are fast, energy-dependent and lectin-inhibited}, author={Guido Middeler and Katharina Zerf and Siegfried Jenovai and Anja Th{\"{u}lig and Mathias Tsch{\"{o}drich-Rotter and Ulrich Kubitscheck and Reiner Peters}, journal={Oncogene}, year={1997}, volume={14}, pages={1407-1417} }