The transport of vitamin B12 through polarized monolayers of Caco-2 cells.

@article{Dix1990TheTO,
  title={The transport of vitamin B12 through polarized monolayers of Caco-2 cells.},
  author={C J Dix and Ibrahim F Hassan and H Y Obray and Rahul Shah and Glynn Wilson},
  journal={Gastroenterology},
  year={1990},
  volume={98 5 Pt 1},
  pages={1272-9}
}
Caco-2 cells grown on 0.45-micron filters, in Millicell chambers, form intact monolayers with many of the properties of polarized intestinal epithelial cells. It is reported here that these cells bind and internalize intrinsic factor-cobalamin complexes and that after 14-28 days in culture this specific binding is exclusively located on the apical membrane. Caco-2 cells also synthesize and secrete a protein with properties similar to transcobalamin II. This protein is secreted from the… CONTINUE READING