The transmission interface of the Saccharomyces cerevisiae multidrug transporter Pdr5: Val-656 located in intracellular loop 2 plays a major role in drug resistance.

@article{Downes2013TheTI,
  title={The transmission interface of the Saccharomyces cerevisiae multidrug transporter Pdr5: Val-656 located in intracellular loop 2 plays a major role in drug resistance.},
  author={Marianne T. Downes and Jitender Mehla and Neeti Ananthaswamy and Adina Wakschlag and Micheala Lamonde and Elliot Dine and Suresh V Ambudkar and John E. Golin},
  journal={Antimicrobial agents and chemotherapy},
  year={2013},
  volume={57 2},
  pages={1025-34}
}
Pdr5 is a major ATP-binding cassette (ABC) multidrug transporter regarded as the founding member of a fungal subfamily of clinically significant efflux pumps. When these proteins are overexpressed, they confer broad-spectrum ultraresistance. To better understand the evolution of these proteins under selective pressure, we exposed a Saccharomyces cerevisiae yeast strain already overexpressing Pdr5 to a lethal concentration of cycloheximide. This approach gave mutations that confer greater… CONTINUE READING
Related Discussions
This paper has been referenced on Twitter 1 time. VIEW TWEETS

References

Publications referenced by this paper.
Showing 1-10 of 26 references

Evidence for a Sav1866-like architecture for the human multidrug transporter P-glycoprotein.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology • 2007
View 4 Excerpts
Highly Influenced

Structural arrangement of the transmission interface in the antigen ABC transport complex TAP.

Proceedings of the National Academy of Sciences of the United States of America • 2009
View 2 Excerpts

A mutation of the H-loop selectively affects rhodamine transport by the yeast multidrug ABC transporter Pdr5.

Proceedings of the National Academy of Sciences of the United States of America • 2008
View 2 Excerpts

Similar Papers

Loading similar papers…