The transmembrane electrochemical gradient of Na+ as driving force for methanol oxidation in Methanosarcina barkeri.

@article{Mller1988TheTE,
  title={The transmembrane electrochemical gradient of Na+ as driving force for methanol oxidation in Methanosarcina barkeri.},
  author={Volker M{\"u}ller and Michael Blaut and Gerhard Gottschalk},
  journal={European journal of biochemistry},
  year={1988},
  volume={172 3},
  pages={601-6}
}
A sodium ion gradient (inside low) across the cytoplasmic membrane of Methanosarcina barkeri was required for methanogenesis from methanol. This could be concluded from the following results. (a) Inhibition of the Na+/H+ antiporter by K+ or amiloride led to an inhibition of methanogenesis from methanol. (b) Upon addition of the sodium ionophore monensin the Na+ gradient was abolished and at the same time methanogenesis from methanol was inhibited. (c) Methanogenesis was impaired when the Na… CONTINUE READING