The transcription initiation sites of eggplant latent viroid strands map within distinct motifs in their in vivo RNA conformations.

@article{LpezCarrasco2016TheTI,
  title={The transcription initiation sites of eggplant latent viroid strands map within distinct motifs in their in vivo RNA conformations.},
  author={Amparo L{\'o}pez-Carrasco and Selma P. Gago-Zachert and Giuseppe Mileti and Sof{\'i}a Minoia and Ricardo Flores and Sonia Delgado},
  journal={RNA biology},
  year={2016},
  volume={13 1},
  pages={83-97}
}
Eggplant latent viroid (ELVd), like other members of family Avsunviroidae, replicates in plastids through a symmetric rolling-circle mechanism in which elongation of RNA strands is most likely catalyzed by a nuclear-encoded polymerase (NEP) translocated to plastids. Here we have addressed where NEP initiates transcription of viroid strands. Because this step is presumably directed by sequence/structural motifs, we have previously determined the conformation of the monomeric linear (+) and… CONTINUE READING