The toolbox of vesicle sidedness determination.

@article{Mszaros2012TheTO,
  title={The toolbox of vesicle sidedness determination.},
  author={P. M{\'e}szaros and D. Hoekstra and J. Kok},
  journal={Analytical biochemistry},
  year={2012},
  volume={429 2},
  pages={
          89-91
        }
}
  • P. Mészaros, D. Hoekstra, J. Kok
  • Published 2012
  • Biology, Medicine
  • Analytical biochemistry
  • Vesicles prepared from cellular plasma membranes are widely used in science for different purposes. The outer membrane leaflet differs from the inner membrane leaflet of the vesicle, and during vesicle preparation procedures two types of vesicles will be generated: right-side-out vesicles, of which the outer leaflet is topologically equivalent to the outer monolayer of the cellular plasma membrane, and inside-out vesicles. Because two populations of vesicles exist, sidedness information of the… CONTINUE READING
    4 Citations

    Tables and Topics from this paper.

    References

    SHOWING 1-10 OF 18 REFERENCES
    Measurement of sidedness of isolated plasma-membrane vesicles: quantitation of actin exposure by DNase I inactivation.
    • 11
    P-Glycoprotein kinetics measured in plasma membrane vesicles and living cells.
    • 49
    Partial purification of a lipoprotein with 5'-nucleotidase activity from membranes of rat liver cells.
    • C. Widnell, J. Unkeless
    • Chemistry, Medicine
    • Proceedings of the National Academy of Sciences of the United States of America
    • 1968
    • 167
    • PDF