Arachidonic acid metabolites, some of which may activate thromboxane A(2) receptors (TPr) and contribute to the development of diabetes complications, including nephropathy, are elevated in diabetes. This study determined the effect of blocking TPr with S18886 or inhibiting cyclooxygenase with aspirin on oxidative stress and the early stages of nephropathy… (More)
FIG. 1. S18886, but not aspirin, attenuates microalbuminuria, glomerular hypertrophy, extracellular matrix deposition, and TGF- 1 expression deposition in diabetic (DM) ApoE / mice. A: Microalbuminuria. Urine samples were collected during the last week of the treatment and used to determine albumin and creatinine concentrations. Data are means SE, n 14–22. B: Glomerular tuft size. Renal sections were stained with Masson’s trichrome stain. Glomerular tuft size was measured on computer-digitalized images. Data are means SE, n 7 for each group (each value represents the average of at least 100 glomeruli from one renal section). C: Extracellular matrix deposition was evaluated semiquantitatively based on the proportion of glomeruli with obvious thickness and matrix deposition at glomerular basement membranes versus total glomeruli counted from four nonoverlapping fields of each renal section under microscope. Data are means SE, n 5 for each group. D: Staining for TGF- 1 was evaluated by semiquantitative scoring. Data are means SE, n 4 for each group. Statistical analysis by ANOVA. ns, not significant.