The thioflavin T fluorescence assay for amyloid fibril detection can be biased by the presence of exogenous compounds.

@article{Hudson2009TheTT,
  title={The thioflavin T fluorescence assay for amyloid fibril detection can be biased by the presence of exogenous compounds.},
  author={Sean A Hudson and Heath Ecroyd and Tak W. Kee and John A Carver},
  journal={The FEBS journal},
  year={2009},
  volume={276 20},
  pages={5960-72}
}
Thioflavin T (ThT) dye fluorescence is used regularly to quantify the formation and inhibition of amyloid fibrils in the presence of anti-amyloidogenic compounds such as polyphenols. However, in this study, it was shown, using three polyphenolics (curcumin, quercetin and resveratrol), that ThT fluorescence should be used with caution in the presence of such exogenous compounds. The strong absorptive and fluorescent properties of quercetin and curcumin were found to significantly bias the ThT… CONTINUE READING

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The thioflavin T fluorescence assay for amyloid fibril detection can be biased by the presence of exogenous compounds

  • S. A. Hudson, H. Ecroyd, T. W. Kee, J. A. Carver
  • Febs Journal
  • 2009

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