The thermosome from Methanopyrus kandleri possesses an NH4+-dependent ATPase activity.

@article{Andrae1998TheTF,
  title={The thermosome from Methanopyrus kandleri possesses an NH4+-dependent ATPase activity.},
  author={S Andrae and G. Frey and R. C. A. Jaenicke and K. O. Stetter},
  journal={European journal of biochemistry},
  year={1998},
  volume={255 1},
  pages={93-9}
}
The ATPase activity of the thermosome from a methanogen, Methanopyrus kandleri, was characterized in detail. In contrast to all other known chaperonins, enzymatic ATP hydrolysis was found to be strictly dependent on high levels of ammonium salts in vitro. The ths gene encoding the thermosome subunit from the hyperthermophilic M. kandleri was functionally expressed in Escherichia coli and the overproduced polypeptide was assembled into intact thermosome complexes in the mesophilic host. The… CONTINUE READING