The thermodynamics of hapten and antigen binding by rabbit anti-dinitrophenyl antibody.

@article{Halsey1975TheTO,
  title={The thermodynamics of hapten and antigen binding by rabbit anti-dinitrophenyl antibody.},
  author={John F. Halsey and Rodney L. Biltonen},
  journal={Biochemistry},
  year={1975},
  volume={14 4},
  pages={800-4}
}
Rabbit anti-dinitrophenyl antibody from a serum pool was obtained as five fractions of purified specific antibody by a limiting antigen precipitation method. Each fraction had a different binding affinity for epsilon-N-2,4-dinitrophenyl-L-lysine. The free energy changes for hapten binding to the five antibody fractions varied from -8.35 to -10.0 kcal/mol… CONTINUE READING