The tenth membrane region of band 3 is initially exposed to the luminal side of the endoplasmic reticulum and then integrated into a partially folded band 3 intermediate.

@article{Kanki2002TheTM,
  title={The tenth membrane region of band 3 is initially exposed to the luminal side of the endoplasmic reticulum and then integrated into a partially folded band 3 intermediate.},
  author={Tomotake Kanki and Masao Sakaguchi and Akiko Kitamura and Takashi Sato and Katsuyoshi Mihara and Naotaka Hamasaki},
  journal={Biochemistry},
  year={2002},
  volume={41 47},
  pages={13973-81}
}
Band 3 is a typical polytopic membrane protein that mediates anion exchange activity [anion exchanger 1 (AE1)]. Although the topology and topogenesis of approximately 40 residues just after transmembrane (TM) 9 have been extensively studied, the topogenesis of this region [tenth region (10thR)] has been unclear. Glycosylation sites created in the 10thR were efficiently glycosylated in a cell-free transcription/translation system, whereas the glycosylation efficiencies were quite low in a… CONTINUE READING