The tRNA-modifying function of MnmE is controlled by post-hydrolysis steps of its GTPase cycle

@inproceedings{Prado2013TheTF,
  title={The tRNA-modifying function of MnmE is controlled by post-hydrolysis steps of its GTPase cycle},
  author={Silvia Prado and Magda Villarroya and Milagros Medina and M.-Eugenia Armengod},
  booktitle={Nucleic acids research},
  year={2013}
}
MnmE is a homodimeric multi-domain GTPase involved in tRNA modification. This protein differs from Ras-like GTPases in its low affinity for guanine nucleotides and mechanism of activation, which occurs by a cis, nucleotide- and potassium-dependent dimerization of its G-domains. Moreover, MnmE requires GTP hydrolysis to be functionally active. However, how GTP hydrolysis drives tRNA modification and how the MnmE GTPase cycle is regulated remains unresolved. Here, the kinetics of the MnmE GTPase… CONTINUE READING