The synthesis of influenza virus negative-strand RNA takes place in insoluble complexes present in the nuclear matrix fraction.

@article{LpezTuriso1990TheSO,
  title={The synthesis of influenza virus negative-strand RNA takes place in insoluble complexes present in the nuclear matrix fraction.},
  author={J A L{\'o}pez-Turiso and Concepci{\'o}n Reynaldo Mart{\'i}nez and Toshinori Endo Soichi Ogishima Hiroshi Tanaka and Juan Ort{\'i}n},
  journal={Virus research},
  year={1990},
  volume={16 3},
  pages={325-37}
}
The replication of influenza virus RNA in vitro has been studied by cell fractionation of MDCK-infected cells and characterization of in vitro synthesized RNA. Analysis of the RNA product polarity by liquid hybridization to excess single-stranded DNA probes shows that only the RNP complexes present in the nuclear matrix fraction are able to synthesize negative-polarity RNA. This RNA product has been characterized as authentic vRNA by size analysis, RNase-protection by unlabelled, positive… CONTINUE READING
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