The synthesis of 4-methylumbelliferyl phenylphosphonate and its used in an improved method for the zymogram analysis of phosphodiesterase I.

@article{Hawley1983TheSO,
  title={The synthesis of 4-methylumbelliferyl phenylphosphonate and its used in an improved method for the zymogram analysis of phosphodiesterase I.},
  author={D. Hawley and M. Crisp and M. E. Hodes},
  journal={Analytical biochemistry},
  year={1983},
  volume={129 2},
  pages={
          522-8
        }
}
  • D. Hawley, M. Crisp, M. E. Hodes
  • Published 1983
  • Chemistry, Medicine
  • Analytical biochemistry
  • The 4-methylumbelliferyl analog of p-nitrophenyl phenylphosphonate was prepared and its cleavage by different phosphohydrolases was studied. The new compound, 4-methylumbelliferyl phenylphosphonate, is hydrolyzed by purified phosphodiesterase I (EC 3.1.4.1) from snake venom or bovine intestinal mucosa. This fact has been exploited to develop a zymogram method which allows the detection of these phosphohydrolases on gels, even when the enzymes are present in small amounts in crude extracts. This… CONTINUE READING
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