The synthesis and characterization of a nuclear membrane affinity chromatography column for the study of human breast cancer resistant protein (BCRP) using nuclear membranes obtained from the LN-229 cells.

@article{Habicht2013TheSA,
  title={The synthesis and characterization of a nuclear membrane affinity chromatography column for the study of human breast cancer resistant protein (BCRP) using nuclear membranes obtained from the LN-229 cells.},
  author={K-L Habicht and Christopher Frazier and Namrata Singh and Ruth Shimmo and Irving W Wainer and Ruin Moaddel},
  journal={Journal of pharmaceutical and biomedical analysis},
  year={2013},
  volume={72},
  pages={159-62}
}
BCRP expression has been reported in glioblastoma cell lines and clinical specimens and has been shown to be expressed both in purified nuclei and in the soluble cytoplasmic fraction. To date, the nuclear BCRP has not been characterized. Our laboratory has previously developed an online chromatographic approach for the study of binding interactions between… CONTINUE READING