The synthesis, testing and use of 5-fluoro-alpha-D-galactosyl fluoride to trap an intermediate on green coffee bean alpha-galactosidase and identify the catalytic nucleophile.

@article{Ly2000TheST,
  title={The synthesis, testing and use of 5-fluoro-alpha-D-galactosyl fluoride to trap an intermediate on green coffee bean alpha-galactosidase and identify the catalytic nucleophile.},
  author={Hoa D. Ly and Shavonne Howard and K Shum and Shouming He and Aiping Zhu and Stephen G Withers},
  journal={Carbohydrate research},
  year={2000},
  volume={329 3},
  pages={539-47}
}
5-Fluoro-alpha-D-galactopyranosyl fluoride was synthesized and its interaction with the active site of an alpha-galactosidase from green coffee bean (Coffea arabica), a retaining glycosidase, characterized kinetically and structurally. The compound behaves as an apparently tight binding (Ki = 600 nM) competitive inhibitor, achieving this high affinity… CONTINUE READING