The synaptophysin/synaptobrevin interaction critically depends on the cholesterol content.

@article{Mitter2003TheSI,
  title={The synaptophysin/synaptobrevin interaction critically depends on the cholesterol content.},
  author={Diana Mitter and Clemens Reisinger and Britta Hinz and Susanne Hollmann and Sowmya V. Yelamanchili and Stephanie Treiber-Held and Thomas Georg Ohm and Andreas Herrmann and Gudrun Ahnert-Hilger},
  journal={Journal of neurochemistry},
  year={2003},
  volume={84 1},
  pages={35-42}
}
Synaptophysin interacts with synaptobrevin in membranes of adult small synaptic vesicles. The synaptophysin/synaptobrevin complex promotes synaptobrevin to built up functional SNARE complexes thereby modulating synaptic efficiency. Synaptophysin in addition is a cholesterol-binding protein. Depleting the membranous cholesterol content by filipin or beta-methylcyclodextrin (beta-MCD) decreased the solubility of synaptophysin in Triton X-100 with less effects on synaptobrevin. In small synaptic… CONTINUE READING