The synaptic vesicle cycle.

@article{Sudhof2004TheSV,
  title={The synaptic vesicle cycle.},
  author={Thomas Sudhof},
  journal={Annual review of neuroscience},
  year={2004},
  volume={27},
  pages={
          509-47
        }
}
  • T. Sudhof
  • Published 2004
  • Biology
  • Annual review of neuroscience
Neurotransmitter release is mediated by exocytosis of synaptic vesicles at the presynaptic active zone of nerve terminals. To support rapid and repeated rounds of release, synaptic vesicles undergo a trafficking cycle. The focal point of the vesicle cycle is Ca2+-triggered exocytosis that is followed by different routes of endocytosis and recycling. Recycling then leads to the docking and priming of the vesicles for another round of exo- and endocytosis. Recent studies have led to a better… 
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References

SHOWING 1-10 OF 207 REFERENCES
Cycling of Synaptic Vesicles: How Far? How Fast!
TLDR
This review attempts to provide an overview of the components involved in both tracks of vesicle cycling, as well as to identify possible mechanistic links between these two pathways.
Distinct pools of synaptic vesicles in neurotransmitter release
TLDR
It is shown that clusters of vesicles at synaptic release sites are composed of two pools, a distal pool containing synapsin and a proximal pool devoid of synAPSin and located adjacent to the presynaptic membrane.
Single and multiple vesicle fusion induce different rates of endocytosis at a central synapse
TLDR
Capacitance measurements are used to resolve the fusion and retrieval of single and multiple vesicles following mild physiological stimulation at a mammalian central synapse and suggest a potential role of regulation of endocytosis in short-term synaptic depression.
Role of Drosophila Rab5 during endosomal trafficking at the synapse and evoked neurotransmitter release
TLDR
It is shown that in conditions in which the synaptic vesicles pool is depleted, the endosome is also drastically reduced and only recovers from membrane derived by dynamin-mediated endocytosis, which suggests that membrane exchange takes place between the vesicle pool and the synaptic endosomes.
The presynaptic cytomatrix of brain synapses
TLDR
This review will review the present view of how the exocytotic and endocytic machineries at the site of neurotransmitter release are linked to and organized by the presynaptic cytoskeleton and summarize recent progress that has been made in understanding how active zones are assembled during nervous system development.
Three modes of synaptic vesicular recycling revealed by single-vesicle imaging
TLDR
It is shown that an exocytic event can terminate with three modes of vesicle retrieval: a fast ‘kiss-and-run’ mode that has a selective fusion pore; a slow (8–21 s) ‘compensatory” mode; and a ‘stranded’ modes of recycling, in which a vesicles is left on the cell surface until a nerve impulse triggers its retrieval.
Accessory factors in clathrin-dependent synaptic vesicle endocytosis
TLDR
A review of advances with a special focus on accessory factors and highlights new aspects of clathrin-mediated endocytosis revealed by the study of these factors.
RIM1α forms a protein scaffold for regulating neurotransmitter release at the active zone
TLDR
It is shown that RIM1α, an active zone protein that was identified as a putative effector for the synaptic vesicle protein Rab3A, interacts with several active zone molecules, including Munc13-1 and α-liprins, to form a protein scaffold in the presynaptic nerve terminal that controls neurotransmitter release.
The small GTP-binding protein Rab3A regulates a late step in synaptic vesicle fusion
TLDR
The precise point at which Rab3A acts in the sequence of synaptic vesicle docking and fusion is determined by using electrophysiological analysis of neurotransmitter release in Rab2A-deficient mice, and the size of the readily releasable pool of vesicles is normal, whereas Ca2+-triggered fusion is altered.
The synaptic vesicle cycle: exocytosis and endocytosis in Drosophila and C. elegans
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