The superoxide-generating system of human neutrophils possesses a novel diaphorase activity. Evidence for distinct regulation of electron flow within NADPH oxidase by p67-phox and p47-phox.

@article{Cross1994TheSS,
  title={The superoxide-generating system of human neutrophils possesses a novel diaphorase activity. Evidence for distinct regulation of electron flow within NADPH oxidase by p67-phox and p47-phox.},
  author={Andrew R. Cross and J L Yarchover and John T. Curnutte},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 34},
  pages={21448-54}
}
A dye reductase activity, independent of the production of superoxide, is induced in membranes prepared from stimulated human neutrophils or during activation of NADPH oxidase in a cell-free system. This diaphorase activity was greater under anaerobic as opposed to aerobic conditions. The activity has an absolute requirement for the membrane components of the oxidase, but does not appear to have an absolute dependence for the 47-kDa cytosolic factor p47-phox, suggesting the oxidase can be… CONTINUE READING