The sulphorhodamine (SRB) assay and other approaches to testing plant extracts and derived compounds for activities related to reputed anticancer activity.

@article{Houghton2007TheS,
  title={The sulphorhodamine (SRB) assay and other approaches to testing plant extracts and derived compounds for activities related to reputed anticancer activity.},
  author={Peter John Houghton and R Yi-Ping Fang and Isariya Techatanawat and Glyn B Steventon and Peter J. Hylands and Clarissa C. Lee},
  journal={Methods},
  year={2007},
  volume={42 4},
  pages={377-87}
}
Since the major approach in searching for potential anticancer agents over the last 50 years has been based on selective cytotoxic effects on mammalian cancer cell lines, cell-based methods for cytotoxicity are described and compared. The sulphorhodamine B (SRB) assay is described in detail as the preferred method and also a novel approach has been developed which is based on the hypothesis that, in some circumstances, the naturally occurring compounds act as prodrugs rather than active… CONTINUE READING