The sugar-specific outer membrane channel ScrY contains functional characteristics of general diffusion pores and substrate-specific porins.

@article{Schlein1991TheSO,
  title={The sugar-specific outer membrane channel ScrY contains functional characteristics of general diffusion pores and substrate-specific porins.},
  author={K. Sch{\"u}lein and Katharina Schmid and R Benzl},
  journal={Molecular microbiology},
  year={1991},
  volume={5 9},
  pages={2233-41}
}
Escherichia coli K-12 strain PS1-28-37 carries the multicopy plasmid pPSO28-37 containing a DNA fragment coding for two of the proteins that enable bacteria to utilize sucrose as sole carbon source. One of the different gene products of the plasmid is the outer membrane protein, ScrY. This protein was isolated and purified by chromatography across a gel filtration column. Reconstitution experiments with lipid bilayer membrane demonstrated that ScrY formed ion-permeable channels with properties… CONTINUE READING

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