The study of PSA gene expression on urogenital cell lines.

  title={The study of PSA gene expression on urogenital cell lines.},
  author={Tohru Takahashi and Senji Hoshi and Makoto Satoh and Toru Kaneda and Ken ichi Suzuki and K i Nakagawara and Seiichi Orikasa},
  journal={International journal of urology : official journal of the Japanese Urological Association},
  volume={6 10},
PURPOSE Reverse transcriptase-polymerase chain reaction (RT-PCR) offers a potentially more sensitive assay for detecting cells expressing prostate-specific antigen (PSA) mRNA in peripheral circulation. But the sensitivity and specificity are variable depending on the position of the PSA amplification. To increase sensitivity and specificity, the whole PSA cDNA (1466 bp) was separated into eight different parts. METHODS We examined RT-PCR on 12 urogenital cell lines, including three prostate… CONTINUE READING

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