The structures of E. coli NfsA bound to the antibiotic nitrofurantoin; to 1,4-benzoquinone and to FMN

@article{Day2021TheSO,
  title={The structures of E. coli NfsA bound to the antibiotic nitrofurantoin; to 1,4-benzoquinone and to FMN},
  author={Martin Day and David Jarrom and Andrew Joseph Christofferson and Antonio E. Graziano and J. L. Ross Anderson and Peter F. Searle and Eva I Hyde and Scott White},
  journal={Biochemical Journal},
  year={2021},
  volume={478},
  pages={2601 - 2617}
}
NfsA is a dimeric flavoprotein that catalyses the reduction in nitroaromatics and quinones by NADPH. This reduction is required for the activity of nitrofuran antibiotics. The crystal structure of free Escherichia coli NfsA and several homologues have been determined previously, but there is no structure of the enzyme with ligands. We present here crystal structures of oxidised E. coli NfsA in the presence of several ligands, including the antibiotic nitrofurantoin. Nitrofurantoin binds with… 
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