The structure-specific endonuclease Ercc1-Xpf is required for targeted gene replacement in embryonic stem cells.

@article{Niedernhofer2001TheSE,
  title={The structure-specific endonuclease Ercc1-Xpf is required for targeted gene replacement in embryonic stem cells.},
  author={Laura J. Niedernhofer and Jeroen Essers and Geert Weeda and Berna H. Beverloo and Jan de Wit and Manja Muijtjens and Hanny Odijk and Jan H. J. Hoeijmakers and Roland Kanaar},
  journal={The EMBO journal},
  year={2001},
  volume={20 22},
  pages={6540-9}
}
The Ercc1-Xpf heterodimer, a highly conserved structure-specific endonuclease, functions in multiple DNA repair pathways that are pivotal for maintaining genome stability, including nucleotide excision repair, interstrand crosslink repair and homologous recombination. Ercc1-Xpf incises double-stranded DNA at double-strand/single-strand junctions, making it an ideal enzyme for processing DNA structures that contain partially unwound strands. Here we demonstrate that although Ercc1 is dispensable… CONTINUE READING

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