The structure of vitamin B12. I. An outline of the crystallographic investigation of vitamin B12

@article{Hodgkin1957TheSO,
  title={The structure of vitamin B12. I. An outline of the crystallographic investigation of vitamin B12},
  author={Dorothy Mary Crowfoot Hodgkin and Jennifer Kamper and June Lindsey and Maureen F. Mackay and Jenny Pickworth and John H. Robertson and Clara Brink-Shoemaker and JOHN G. White and RICHARD J. Prosen and Kenneth N. Trueblood},
  journal={Proceedings of the Royal Society of London. Series A. Mathematical and Physical Sciences},
  year={1957},
  volume={242},
  pages={228 - 263}
}
  • D. Hodgkin, J. Kamper, K. Trueblood
  • Published 29 October 1957
  • Chemistry
  • Proceedings of the Royal Society of London. Series A. Mathematical and Physical Sciences
The structure of vitamin B12 has been examined by the X-ray analysis of four different crystal structures, air-dried and wet vitamin B 12, the selenocyanate derivative of B12, and a hexacarboxylic acid prepared by the degradation of the vitamin by Cannon, Johnson and Todd. The analysis turned on the possibility of identifying some of the atomic positions in the molecule in very confused appproximate electron density distributions calculated using only evidence of cobalt or cobalt and selenium… 

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References

SHOWING 1-10 OF 10 REFERENCES
Vitamin B12: the identification of vitamin B12 as a cyano-cobalt coordination complex.
Science, founded in 1880, is published each Friday by the American Association for the Advancement of Science at the Business Press, 10 McGovern Ave., Lancaster, Pa. Entered as second-class matter at
Purification of anti-pernicious anaemia factors from liver.
TLDR
From ox liver two red pigments both highly active in pernicious anaemia are prepared, and the proportions suggest that one arises from the other by proteolysis.