The structure of the cytoplasm of lens fibers as determined by conical tomography.

@article{Schietroma2009TheSO,
  title={The structure of the cytoplasm of lens fibers as determined by conical tomography.},
  author={Cataldo Schietroma and N. H. Fain and Lorenzo M. Zampighi and Salvatore Lanzavecchia and Guido A. Zampighi},
  journal={Experimental eye research},
  year={2009},
  volume={88 3},
  pages={566-74}
}
Studies using conventional electron microscopy describe the cytoplasm of lens fiber cells as having essentially an amorphous structure. We hypothesized that significant structural detail might have been lost as a result of projecting the entire thickness of the section (50-100 nm) onto a single plane (the "projection artifact"). To test this hypothesis, we studied the 3D-structure of rat lens cortical fibers before and after extracting the "soluble" crystallins with low ionic strength buffers… CONTINUE READING