The structure of the Escherichia coli hemB gene.

  title={The structure of the Escherichia coli hemB gene.},
  author={J M Li and Charlotte Sananes Russell and Sharon D. Cosloy},
  volume={75 1},
The Escherichia coli hemB gene, which encodes 5-aminolevulinic acid dehydratase, and was cloned into pTZ18U, a multicopy plasmid, was sequenced. The hemB insert was double-digested with restriction enzymes and recloned back into pTZ18U and pTZ19U to allow for sequencing in two directions. In a second procedure, used to fill in gaps and to confirm the sequence derived from the first procedure, the whole insert was cloned into M13 phages. A nested set of deletions was constructed and recloned… CONTINUE READING