# The structure of one of the eight or more distinct chromosomal genes for human interferon-α

@article{Nagata1980TheSO,
title={The structure of one of the eight or more distinct chromosomal genes for human interferon-$\alpha$},
author={Shigekazu Nagata and Ned Mantei and Charles Weissmann},
journal={Nature},
year={1980},
volume={287},
pages={401-408}
}
• Published 2 October 1980
• Biology, Medicine
• Nature
The 12 Interferon (IFN)-related sequences detected in a human gene bank fall into not less than eight distinct classes, indicating that there are at least eight IFN-related genes. Most, if not all, of these direct the synthesis of an IFN in Escherichia coli. The sequence of one chromosomal gene and its flanking regions was identical to that deduced for the cDNA corresponding to IFN-αl mRNA. No evidence was found for the existence of an intron, in either the coding or the non-coding segments of… Expand

#### Topics from this paper

Structure of the human immune interferon gene
• Biology, Medicine
• Nature
• 1982
All the evidence to date and the present data suggest that this is the only gene for IFN-γ and that the resolution of IFn-γ into two components18 is probably the result of post-translational processing of the protein. Expand
The Structure and Bacterial Expression of Three Distinct Bovine Interferon-β Genes
• Biology
• Bio/Technology
• 1984
Southern hybridization analysis indicates the presence of at least five members in the bovine IFN-β gene family and DNA sequence analysis demonstrates that these genes contain no introns and code for homologous, yet distinct, proteins. Expand
Nucleotide sequence of the chromosomal gene for human fibroblast (beta 1) interferon and of the flanking regions.
• Biology, Medicine
• Gene
• 1981
The nucleotide sequence of the human fibroblast (beta 1) interferon chromosomal gene and its flanking regions was determined. These results confirm the absence of intervening sequences in the gene.Expand
Structural relationship of human interferon alpha genes and pseudogenes.
The finding that at least two IFN-alpha genes appear to be natural hybrids of other IFN -alpha genes, and that two distinct IFn-alpha loci have completely identical coding sequences, although their flanking regions are different, is evidence for information exchange between the individual genes. Expand
Molecular analysis of the human interferon-α gene family
• Biology
• 1981
It is concluded that there exist at least 11 distinct genes of gene-like sequences of the interferon-alpha type in the human genome, of which most likely represents an allelic variant, and at least five pseudogenes distantly related to the interFERon- alpha genes. Expand
Human Immune Interferon (IFN-γ) Gene Sequence and Structure
• Biology
• 1983
This gene has been isolated from a recombinant human-λ gene library, and the entire gene and 5′ and 3′ flanking regions have been sequenced. Expand
Partial Mapping of Ten Genes of the Human Interferon- α Family
• Biology
• 1981
ABSTRACT HuIFN-α genes form a family of not less than 9–10 distinct members, at least three of which are arranged in tandem. In one case, two variants, believed to be alleles of the same gene, couldExpand
Identification of a functional allele of a human interferon-alpha gene previously characterized as a pseudogene.
• Biology, Medicine
• Journal of interferon research
• 1989
Three recombinant phage lambda L47 clones containing 4 alpha interferon (IFN) genes have been isolated from a newly constructed human genomic library and it appears likely that other variant alleles may remain to be described and that the IFN system may be able to tolerate some degeneracy as a consequence of the large number of members of the family. Expand
Novel human leukocyte interferon subtype and structural comparison of alpha interferon genes.
• Biology, Medicine
• Journal of interferon research
• 1984
A comparison of nucleotide sequences within the genes coding for different subtypes of human leukocyte interferon (IFN-alpha) has revealed the natural hybrid structure of individual alpha-IFNs-H,B,F, and N. Expand
Efficient expression in Escherichia coli of two species of human interferon-alpha and their hybrid molecules.
• Biology, Medicine
• DNA
• 1985
An expression plasmid which directs the synthesis of the mature IFN-alpha I' peptide was constructed using vectors carrying the lpp/lac promoter and "runaway" replicon and the specific antiviral activities of the hybrid IFNs on human and bovine cells were similar to that of the amino-terminal parental IFN peptide. Expand

#### References

SHOWING 1-10 OF 52 REFERENCES
The chromosomal arrangement of human α-like globin genes: Sequence homology and α-globin gene deletions
• Biology
• Cell
• 1980
Analysis of the cloned DNA confirms the linkage arrangement of the two adult α-globin genes (α1 and α2) previously derived from genomic blotting experiments and identifies two additional closely linked α-like genes. Expand
Synthesis in E. coli of a polypeptide with human leukocyte interferon activity
Double-stranded cDNA prepared from the 12S fraction of poly (A) RNA from interferon (IF)-producing human leukocytes was cloned in Escherichia coli using the pBR322 vector. One of the resulting clonesExpand
The structure and evolution of the two nonallelic rat preproinsulin genes
• Medicine, Biology
• Cell
• 1979
Calculations based on the divergence of the two preproinsulin nucleotide and amino acid sequences indicate that these genes are the products of a recent duplication. Expand
The structure and transcription of four linked rabbit β-like globin genes
• Biology
• Cell
• 1979
Determination of the nucleotide sequence of gene β1 confirms that this gene corresponds to the second type of two common co-dominant alleles encoding the adult β-globin chain, and partial DNA sequence analysis indicates that the large intron in β4 is located between codons for amino acids lysine and leucine in an analogous position to that of the largeintron inβ1. Expand
Human leukocyte and fibroblast interferons are structurally related
• Biology, Medicine
• Nature
• 1980
The coding sequences of the cDNAs of cloned human leukocyte interferon I and human fibroblast interferon show homologies of 45% at the nucleotide and 29% at the amino acid level. We conclude that theExpand
The isolation and characterization of linked δ- and β-globin genes from a cloned library of human DNA
• Biology
• Cell
• 1978
The two independently isolated β-globin clones differ from each other by the presence of a Pst I restriction enzyme cleavage site within the large intervening sequence of the δ- globin gene of one of the clones, which suggests that the human DNA carried in the two clones was derived from two homologous chromosomes which were heterozygous for the PstI restriction enzyme recognition sequence. Expand
Isolation and structure of a human fibroblast interferon gene
From the nucleotide sequence of the gene, the complete amino acid sequence of human fibroblast interferon was deduced and the protein is 166 amino acids long and is preceded by a 21-amino acid signal sequence. Expand
The nucleotide sequence of human fibroblast interferon cDNA.
• Biology, Medicine
• Gene
• 1980
The data reported predict the hitherto unknown amino acid sequence of human fibroblast interferon and its putative signal peptide. Expand
The nucleotide sequence of a cloned human leukocyte interferon cDNA.
• Biology, Medicine
• Gene
• 1980
The nucleotide sequence of the human leukocyte interferon cDNA carried in hybrid plasmid Z-pBR322(Pst)/HcIF-2h, which has been shown to direct the formation of a polypeptide with human leucocyteinterferon activity, suggests that these two interferons are encoded by two non-allelic genes. Expand
Molecular evolution of human and rabbit beta-globin mRNAs.
• Biology, Medicine
• Proceedings of the National Academy of Sciences of the United States of America
• 1977
It is shown that not all silent mutations in globin mRNA are neutral, and divergent nucleotides tend to be distributed nonrandomly: in the coding region silent substitutions are most rare in segments that are also deficient in substitutions leading to replacements. Expand