The structure of gelsolin bound to ATP.

  title={The structure of gelsolin bound to ATP.},
  author={Dunja Urosev and Qing Ma and Agnes Lay-Choo Tan and Robert C Robinson and Leslie D. Burtnick},
  journal={Journal of molecular biology},
  volume={357 3},
Calcium activation of the actin-modifying properties of gelsolin is sensitive to ATP. Here, we show that soaking calcium-free gelsolin crystals in ATP-containing media results in ATP occupying a site that spans the two pseudosymmetrical halves of the protein. ATP binding involves numerous polar and hydrophobic contacts and is identical for the two copies of gelsolin related by non-crystallographic symmetry within the crystal. The gamma-phosphate of ATP participates in several charge-charge… CONTINUE READING