# The structure of crystalline profilinβ-actin

@article{Schutt1993TheSO,
title={The structure of crystalline profilin$\beta$-actin},
author={Clarence Schutt and James C. Myslik and Michael D. Rozycki and Nalin C. W. Goonesekere and Uno Lindberg},
journal={Nature},
year={1993},
volume={365},
pages={810-816}
}
• Published 28 October 1993
• Chemistry
• Nature
The three-dimensional structure of bovine profilinβ-actin has been solved to 2.55 Å resolution by X-ray crystallography. There are several significant local changes in the structure of β-actin compared with α-actin as well as an overall 5° rotation between its two major domains. Actin molecules in the crystal are organized into ribbons through intermolecular contacts like those found in oligomeric protein assemblies. Profilin forms two extensive contacts with the actin ribbon, one of which…
496 Citations

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• Chemistry
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Applying osmotic pressure to profilin:beta-actin crystals brings about a collapse of the unit cell comparable with that seen in the open to tight-state transition, enabling an estimate of the work required to cause this transformation of beta- actin in the crystals.

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The role of Profilin in actin elongation and its association with the formin protein is discussed and the apicomplexan-specific mini-domain present in profilin is discussed in order to deduce the functional aspect of these domains.

### Crystal packing induces a conformational change in profilin-I from Acanthamoeba castellanii.

• Chemistry
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