The structure of a resuscitation-promoting factor domain from Mycobacterium tuberculosis shows homology to lysozymes

@article{CohenGonsaud2005TheSO,
  title={The structure of a resuscitation-promoting factor domain from Mycobacterium tuberculosis shows homology to lysozymes},
  author={Martin Cohen-Gonsaud and Philippe Barthe and Claire Bagn{\'e}ris and Brian Henderson and John Ward and Christian Roumestand and Nicholas H Keep},
  journal={Nature Structural &Molecular Biology},
  year={2005},
  volume={12},
  pages={270-273}
}
Resuscitation-promoting factor (RPF) proteins reactivate stationary-phase cultures of (G+C)-rich Gram-positive bacteria including the causative agent of tuberculosis, Mycobacterium tuberculosis. We report the solution structure of the RPF domain from M. tuberculosis Rv1009 (RpfB) solved by heteronuclear multidimensional NMR. Structural homology with various glycoside hydrolases suggested that RpfB cleaved oligosaccharides. Biochemical studies indicate that a conserved active site glutamate is… CONTINUE READING