The structure and dynamics of patch-clamped membranes: a study using differential interference contrast light microscopy

@article{Sokabe1990TheSA,
  title={The structure and dynamics of patch-clamped membranes: a study using differential interference contrast light microscopy},
  author={Masahiro Sokabe and Frederick Sachs},
  journal={The Journal of Cell Biology},
  year={1990},
  volume={111},
  pages={599 - 606}
}
We have developed techniques for micromanipulation under high power video microscopy. We have used these to study the structure and motion of patch-clamped membranes when driven by pressure steps. Patch-clamped membranes do not consist of just a membrane, but rather a plug of membrane-covered cytoplasm. There are organelles and vesicles within the cytoplasm in the pipette tip of both cell-attached and excised patches. The cytoplasm is capable of active contraction normal to the plane of the… CONTINUE READING