The structural basis of ribosome activity in peptide bond synthesis.

@article{Nissen2000TheSB,
  title={The structural basis of ribosome activity in peptide bond synthesis.},
  author={Poul Nissen and Jeffrey L Hansen and Nenad Ban and Peter B Moore and Thomas A. Steitz},
  journal={Science},
  year={2000},
  volume={289 5481},
  pages={
          920-30
        }
}
Using the atomic structures of the large ribosomal subunit from Haloarcula marismortui and its complexes with two substrate analogs, we establish that the ribosome is a ribozyme and address the catalytic properties of its all-RNA active site. Both substrate analogs are contacted exclusively by conserved ribosomal RNA (rRNA) residues from domain V of 23S rRNA; there are no protein side-chain atoms closer than about 18 angstroms to the peptide bond being synthesized. The mechanism of peptide bond… Expand
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Mutation of the conserved base A2451 of 23 S rRNA to U did not abolish the pH dependence of the reaction with puromycin in M. smegmatis, suggesting that A24 51 did not confer the pH dependent role, however, the A 2451U mutation alters the structure of the peptidyl transferase center and changes the pattern of pH-dependent rearrangements, as probed by chemical modification of 23S rRNA. Expand
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  • Proceedings of the National Academy of Sciences of the United States of America
  • 2005
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The observed H-bond network suggests an important structural role of several universally conserved rRNA residues, and the catalytic effect is found to be entirely of entropic origin, in accordance with recent experimental data. Expand
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TLDR
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TLDR
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TLDR
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  • Biology, Medicine
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TLDR
Biochemical, genetic and structural evidence highlight the role of the ribosome as an entropic catalyst that accelerates peptide bond formation primarily by substrate positioning and suggests that peptide release should more strongly depend on chemical catalysis likely involving an rRNA group of the PTC. Expand
Ribosomal crystallography: peptide bond formation, chaperone assistance and antibiotics activity.
TLDR
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TLDR
A current understanding of the mechanism of the ribosome-catalyzed peptide bond formation is the focus of this review and implications on the mechanisms of peptide release are discussed. Expand
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