The structural basis of ankyrin function. II. Identification of two functional domains.

@article{Weaver1984TheSB,
  title={The structural basis of ankyrin function. II. Identification of two functional domains.},
  author={D. C. Weaver and G. Pasternack and Vincent T. Marchesi},
  journal={The Journal of biological chemistry},
  year={1984},
  volume={259 10},
  pages={6170-5}
}
Human erythrocyte ankyrin was cleaved by restricted proteolysis at 0 degrees C into two distinct chemical domains. The site on ankyrin that binds spectrin was found to be within a 55,000-dalton domain by spectrin affinity chromatography and co-sedimentation with spectrin in a sucrose gradient. A 32,000-dalton fragment of this domain was prepared (tryptic digest, 0 degrees C, 24 h), separated by gel filtration, and shown to inhibit spectrin binding to the membrane. By comparison with previous… CONTINUE READING
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