The structural basis for pseudoreversion of the H95N lesion by the secondary S96P mutation in triosephosphate isomerase.

Abstract

The structural basis for the 3000-fold decrease in catalytic efficiency of the H95N mutant chicken triosephosphate isomerase and the 60-fold regain of catalytic efficiency in the double mutant, H95N.S96P, have been analyzed. The results from a combination of X-ray crystallography and Fourier transform infrared spectroscopy experiments indicate that the… (More)

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@article{Komives1996TheSB, title={The structural basis for pseudoreversion of the H95N lesion by the secondary S96P mutation in triosephosphate isomerase.}, author={Elizabeth A. Komives and J C Lougheed and Zhenrong Zhang and Shouta Sugio and Nagamani Narayana and N. H. Xuong and Greg A. Petsko and Donald Ringe}, journal={Biochemistry}, year={1996}, volume={35 48}, pages={15474-84} }