The structural and functional reliability of Circulins of Chassalia parvifolia for peptide therapeutic scaffolding

  title={The structural and functional reliability of Circulins of Chassalia parvifolia for peptide therapeutic scaffolding},
  author={Senthilkumar Balaraman and R. Ramalingam},
  journal={Journal of Cellular Biochemistry},
  pages={3999 - 4008}
Computational methods have refined the mode of peptide drug designing to a new plateau recently. Circulin, a 30 residue natural plant polypeptide acts as a plant defensive peptide. Additional to its antimicrobial activity it also possesses an inhibitory cytopathic effect on the replication of human immunodeficiency virus (HIV). Stable Circulin can be a functionally able template for scaffolding peptide based drugs. Hence, structural stability of Chassalia parvifolia, Circulin A (1BH4), and… Expand
2 Citations
Cyclotides: From Structure to Function.
This Review explores the class of plant-derived macrocyclic peptides called cyclotides, characterized by their head-to-tail cyclic backbone and cystine knot motif, which render them to be exceptionally stable, with resistance to thermal or enzymatic degradation. Expand
Cyclotides: disulfide-rich peptide toxins in plants.
A brief overview of cyclotide discovery, characterization, distribution, synthesis and mode of action mechanisms is given to focus on their toxicities to insect pests and their medical and agricultural applications. Expand


In Silico Template Selection of Short Antimicrobial Peptide Viscotoxin for Improving Its Antimicrobial Efficiency in Development of Potential Therapeutic Drugs
It is proposed that viscotoxin A3 (1ED0) could be used as a preeminent template for scaffolding potentially efficient antimicrobial peptide-based drugs and nanomaterials in future. Expand
Solution structure by NMR of circulin A: a macrocyclic knotted peptide having anti-HIV activity.
The three-dimensional solution structure of circulin A, a 30 residue polypeptide from the African plant Chassalia parvifolia, has been determined using two-dimensional 1H-NMR spectroscopy and provides additional evidence for a solvent-exposed Trp residue. Expand
Structure of Circulin B and Implications for Antimicrobial Activity of the Cyclotides
Comparison of cyclotide structures and activities suggests that the presence and location of cationic residues may be a requirement for activity against Gram negative bacteria. Expand
Analysis of the Structural Stability Among Cyclotide Members Through Cystine Knot Fold that Underpins Its Potential Use as a Drug Scaffold
Recent emergence of plant derived peptide cyclotides, characterized with a cyclized head-to-tail backbone and three disulfide bonds forming cyclic cystine knot, has advanced the field ofExpand
In‐Silico Template Selection of In‐Vitro Evolved Kalata B1 of Oldenlandia Affinis for Scaffolding Peptide‐Based Drug Design
It is proposed that the [P20D, V21K] kB1 (2F2I) could be the best template for scaffolding peptide based drug design and supported by the statistically validated contours of polar surface area, hydrogen bond distribution and the distance of disulfide bridges. Expand
Analysis of the disulfide linkage pattern in circulin A and B, HIV-inhibitory macrocyclic peptides.
The disulfide pairing motif of circulin A and circulin B was determined and the cysteine residue pairings were identified by analysis of the complex mixture of cleavage products that resulted from partial acid hydrolysis of the native peptides. Expand
Discovery, structure and biological activities of the cyclotides.
The background to the discovery of the cyclotides, their structural characterization, chemical synthesis, genetic origin, biological activities and potential applications in the pharmaceutical and agricultural industries are described. Expand
An unusual structural motif of antimicrobial peptides containing end-to-end macrocycle and cystine-knot disulfides.
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Structural plasticity of the cyclic-cystine-knot framework: implications for biological activity and drug design.
This finding confirms the tolerance of the cyclotide framework to residue substitutions and opens up possibilities for the substitution of biologically active peptide epitopes into the framework. Expand
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The cyclotides are the largest of several groups of naturally occurring circular proteins that have been discovered in bacteria, plants and animals over recent years and their applications in drug design are described. Expand