The steroid metabolite 16(β)-OH-androstenedione generated by CYP21A2 serves as a substrate for CYP19A1

  title={The steroid metabolite 16($\beta$)-OH-androstenedione generated by CYP21A2 serves as a substrate for CYP19A1},
  author={Jens Neunzig and Mohammed Milhim and Lina Schiffer and Yogan Khatri and Josef Zapp and Alberto S{\'a}nchez-Guijo and Michaela F. Hartmann and Stefan A. Wudy and Rita Bernhardt},
  journal={The Journal of Steroid Biochemistry and Molecular Biology},
Differential effects of variations in human P450 oxidoreductase on the aromatase activity of CYP19A1 polymorphisms R264C and R264H
The studies demonstrate that the allelic variants of P450 when present with a variant form of POR may show different activities, and combined effects of variations in both the P450 enzymes as well as in the POR should be considered when genetic data is available.
Effect of sulfonated steroids on steroidogenic cytochrome P450-dependent steroid hydroxylases
Human cytochrome P450 enzymes 5–51 as targets of drugs and natural and environmental compounds: mechanisms, induction, and inhibition – toxic effects and benefits
This review covers the 22 (of the total of 57) human P450s in Families 5–51 and their substrate selectivity and update and discuss important aspects of each of these 22 P 450s and questions that remain open.
Identification and circumvention of bottlenecks in CYP21A2‐mediated premedrol production using recombinant Escherichia coli
This study successfully improved the whole‐cell process in terms of premedrol production by improving the electron supply to CYP21A2 and circumventing substrate inhibition which is presumed to be the main limiting factor of the presented system by developing an improved fed‐batch protocol.
Novel CYP19A1 Mutations Extend the Genotype-Phenotype Correlation and Reveal the Impact on Ovarian Function
In girls, aromatase deficiency usually manifests at birth, but diagnosis may also be made because of abnormal pubertal development or ovarian torsion due to (poly)cystic ovaries, which puts the ovary harboring CYP19A1 variants at very high risk to produce cysts with aging and is therefore prone to ovarian tORSion.


2β- and 16β-hydroxylase activity of CYP11A1 and direct stimulatory effect of estrogens on pregnenolone formation
A steroidogenic pathway for sulfonated steroids: The metabolism of pregnenolone sulfate
Dehydroepiandrosterone Sulfate (DHEAS) Stimulates the First Step in the Biosynthesis of Steroid Hormones
Findings indicate that DHEAS affects steroid hormone biosynthesis on a molecular level resulting in an increased formation of pregnenolone.
11β-Hydroxyandrostenedione Returns to the Steroid Arena: Biosynthesis, Metabolism and Function
An overview of the research on 11OHA4 since its identification in 1953 will be presented, with specific focus on the most recent works that have advanced the understanding of its biological role, thereby underscoring its relevance in health and disease.
Partial agonist/antagonist properties of androstenedione and 4-androsten-3β,17β-diol
Intraadrenal steroid metabolism in the guinea pig: guinea pig adrenal microsomes metabolize androstenedione in a manner distinct from liver microsomes.
The hydroxylation reactions performed by adrenal tissue are consistent with the presence in adrenal microsomes of immunochemical homologues of members of the CYP1A, 2B, 2C and 3A families which have known steroid hydroxyation functions in liver.
A survey of the high-field 1H NMR spectra of the steroid hormones, their hydroxylated derivatives, and related compounds
1 H NMR chemical shifts are presented for virtually all the protons in 166 steroids. These comprise mainly the hormones testosterone, androst-4-ene-3,17-dione, progesterone, and a wide range of
Development of a non-high pressure liquid chromatography assay to determine testosterone hydroxylase (CYP3A) activity in human liver microsomes.
Tritium release from [1,2,6, 7-3H]testosterone provides a simple and rapid alternative to the HPLC procedure for measuring CYP3A4/5 activity in human liver microsomes, however, the tritium-release assay may have limited value in measuring CYp3A activity in livermicrosomes from other species.
The critical iron-oxygen intermediate in human aromatase.