The spliced structures of adenovirus 2 fiber message and the other late mRNAs

@article{Chow1978TheSS,
  title={The spliced structures of adenovirus 2 fiber message and the other late mRNAs},
  author={Louise T. Chow and Thomas R. Broker},
  journal={Cell},
  year={1978},
  volume={15},
  pages={497-510}
}
All late adenovirus 2 messenger RNAs, except perhaps that for peptide IX, are composite molecules with sequences derived from at least two to five or more separate portions of the genome, as determined by electron microscopic analyses of polysomal RNA hybridized to single- or double-stranded DNA. We previously reported that eight different rightward-transcribed mRNAs contain, at their 5' ends, a common three-part leader sequence derived from coordinates 16.6, 19.6 and 26.6, and that a ninth… 
Structure and metabolism of adenovirus RNAs containing sequences from the fiber gene.
TLDR
This study identified and characterized polyadenylated nuclear RNAs that contain fiber sequences and therefore are possible processing intermediates for adenovirus fiber messenger RNA and results are consistent with a precursor function for the 35 S RNAs and the 85.9 to 96.9 28 S species.
The structure of adenovirus 2 early nuclear and cytoplasmic RNAs.
Abstract Electron microscopy of hybrids formed between the separated DNA strands of human adenovirus type 2 and poly(A)-containing nuclear or cytoplasmic RNA isolated at an early timepoint of
Nucleotide sequence analysis of the leader segments in a cloned copy of adenovirus 2 fiber mRNA
TLDR
This prototype sequence, which has also been recognized at or near the splice points in other eucaryotic systems, is possibly part of a larger unit which serves as a recognition site for specific excision-ligation events that ultimately lead to the production of mature mRNAs.
Sequences from the beginning of the fiber messenger RNA of adenovirus-2.
Small restriction fragments, from around co-ordinate 86.6 on the adenovirus-2 genome, have been used as primers for direct DNA sequence analysis by Sanger's (Sanger et al., 1977) chain termination
In vitro translation products specified by the transforming region of adenovirus type 2
Region 1 DNA sequences (map positions 0 to 11% on the linear adenovirus 2 genome) are expressed both early and late in lytic infection and are required for transformation by the virus. During
Complex splicing patterns of RNAs from the early regions of adenovirus-2.
TLDR
From the large, complex arrays of composite RNA structures, numerous insights into the RNA splicing mechanisms were inferred.
Splice junctions in adenovirus 2 early region 4 mRNAs: multiple splice sites produce 18 to 24 RNAs
TLDR
The splice junctions in adenovirus 2 early region 4 (E4) mRNAs were localized and eight acceptor and two donor splice sites utilized in the deletion of the intron from the 3' portion of these m RNAs were detected.
The Spliced Messenger RNAs of Adenovirus-2
TLDR
A theory is presented which may help to understand the nature and the evolution of RNA splicing, and sequences extremely close to this start point are implicated as the junction between the leader segment and the main body of fiber mRNA.
A supplementary adenoviral leader sequence and its role in messenger translation
TLDR
Fractionation of total poly(A)-containing Ad2 + ND1 dp2 "late" cytoplasmic RNA in methyl mercuric hydroxide-agarose gels established that the presence or absence of the fourth leader sequence has little or no influence on the ability of the messenger to direct the synthesis of the 23–24K dalton protein(s) in a cell-free translation system derived from rabbit reticulocytes.
mRNAs from human adenovirus 2 early region 4
The molecular structure of the mRNAs from early region 4 of human adenovirus 2 has been studied by Northern blot analysis, S1 nuclease analysis, and sequence analysis of cDNA clones. The results make
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Two regions of the adenovirus 2 genome specify families of late polysomal RNAs containing common sequences.
  • M. Mcgrogan, H. Raskas
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1978
TLDR
Late cytoplasmic RNAs specified by two regions of the adenovirus 2 genome were analyzed by size fractionation of poly(A)-[3H]RNA and subsequent hybridization to DNA fragments to allow the translation of multiple polypeptides from a genome segment that has only one terminator site for mRNA synthesis.
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TLDR
Four segments of viral RNA may be joined together during the synthesis of mature hexon mRNA, a model is presented for adenovirus late mRNA synthesis that involves multiple splicing during maturation of a larger precursor nuclear RNA.
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TLDR
Fractionation of total poly(A)-containing Ad2 + ND1 dp2 "late" cytoplasmic RNA in methyl mercuric hydroxide-agarose gels established that the presence or absence of the fourth leader sequence has little or no influence on the ability of the messenger to direct the synthesis of the 23–24K dalton protein(s) in a cell-free translation system derived from rabbit reticulocytes.
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TLDR
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TLDR
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TLDR
Specific fragments of adenovirus type 2 DNA, generated by cleavage with restriction endonucleases endoR, Eco RI,endoR and Hin dIII were used in hybridization-mapping experiments.
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TLDR
It is concluded that the three mRNA's are coterminal in sequence at their 3' ends and overlap at internal positions and implications for the protein-coding potential of these mRNA's and the mechanisms of adenovirus tyep 2 late RNA processing are discussed.
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