The sperm entry site during fertilization of the zebrafish egg: localization of actin.

@article{Hart1992TheSE,
  title={The sperm entry site during fertilization of the zebrafish egg: localization of actin.},
  author={Nathan H. Hart and Kathryn A. Becker and Joseph S. Wolenski},
  journal={Molecular reproduction and development},
  year={1992},
  volume={32 3},
  pages={
          217-28
        }
}
The sperm entry site (SES) of zebrafish (Brachydanio rerio) eggs was studied before and during fertilization by fluorescence, scanning, and transmission electron microscopy. Rhodamine phalloidin (RhPh), used to detect polymerized filamentous actin, was localized to microvilli of the SES and to cytoplasm subjacent to the plasma membrane in the unfertilized egg. The distribution of RhPh staining at the SES correlated with the ultrastructural localization of a submembranous electrondense layer of… CONTINUE READING
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