The specific amino acid requirements of a mammalian cell (strain L) in tissue culture.

@article{Eagle1955TheSA,
  title={The specific amino acid requirements of a mammalian cell (strain L) in tissue culture.},
  author={Harry Eagle},
  journal={The Journal of biological chemistry},
  year={1955},
  volume={214 2},
  pages={
          839-52
        }
}
  • H. Eagle
  • Published 1 June 1955
  • Biology
  • The Journal of biological chemistry

Tables from this paper

ACTIVITY OF VITAMIN B12 IN MAMMALIAN CELLS IN TISSUE CULTURE

In 1956, Evans and reported the development of a chemically defined culture medium for the growth of mammalian tissue cells in witro, which has supported the continuous proliferation of 11 established cell strains from different tissues of the mouse, hamster, monkey, and one of these strains, 2071,3 was a clonal derivative of strain L,738 which originated from the subcutaneous connective tissue of a C3H mouse.

Cultivation of animal cells in chemically defined media, a review.

  • K. Higuchi
  • Biology
    Advances in applied microbiology
  • 1973

MOLECULAR GROWTH REQUIREMENTS OF SINGLE MAMMALIAN CELLS

Under certain conditions, growth of single HeLa cells of the S3 clonal strain requires the presence of cholesterol at a level of 0.5 to 1 microgram per milliliter.

LATENT VIRAL INFECTION OF CELLS IN TISSUE CULTURE

Mouse fibroblasts (L cells) fail to support the growth of psittacosis virus if they are maintained on a medium containing only inorganic salts and glucose for 2 days prior to infection, and L cell cultures maintained on media deficient in phenylalanine or tryptophan for 2 Days before infection were found to be incapable of supporting virus growth.

The free amino acid pool in L-strain fibroblasts.

Glutamine and the regulation of DNA replication and cell multiplication in fibroblasts

Growing and quiescent 3T3‐fibroblasts were exposed to media in which the concentration of the 13 essential amino acids had been lowered to 1/100 or 1/1,000 of that in DMEM ‐ either all together or one by one.

LATENT VIRAL INFECTION OF CELLS IN TISSUE CULTURE

In most cases in which a specific vitamin requirement was shown the decreased yield of virus was correlated with a delay in the cytopathic effects produced in the cell cultures by psittacosis virus, although the possibility of incomplete vitamin depletion of the cells has not been eliminated.
...

References

Biochemistry and Physiology of Nutrition

Biochemistry and Physiology of NutritionVol. 1. Edited By Geoffrey H. Bourne and George W. Kidder. Pp. xiii + 569. (New York: Academic Press, Inc.; London: Academic Books, Ltd., 1953.) 13 dollars.