The slice overlay assay: a versatile tool to study the influence of extracellular signals on neuronal development.

@article{Polleux2002TheSO,
  title={The slice overlay assay: a versatile tool to study the influence of extracellular signals on neuronal development.},
  author={Franck Polleux and Anirvan Ghosh},
  journal={Science's STKE : signal transduction knowledge environment},
  year={2002},
  volume={2002 136},
  pages={pl9}
}
We have developed a technique that allows characterization and identification of extracellular signals that regulate various aspects of neuronal differentiation. In this in vitro assay, dissociated cells isolated from the developing cerebral wall are labeled and cultured over organotypic cortical slices. We have used this slice overlay assay to identify some of the extracellular cues that regulate differentiation and patterning of axons and dendrites in the cerebral cortex. This assay can be… CONTINUE READING

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