The six amino acid antimicrobial peptide bLFcin6 penetrates cells and delivers siRNA.

@article{Fang2013TheSA,
  title={The six amino acid antimicrobial peptide bLFcin6 penetrates cells and delivers siRNA.},
  author={Bing Fang and Hui Y Guo and Ming Zhang and Lu Jiang and Fazheng Ren},
  journal={The FEBS journal},
  year={2013},
  volume={280 4},
  pages={1007-17}
}
Cell-penetrating peptides (CPPs) are a new class of vectors with high pharmaceutical potential to deliver bioactive cargos into cells. Here, we characterized bLFcin(6) , a six amino acid peptide derived from bovine lactoferricin, as a CPP. Uptake of bLFcin(6) was measured by flow cytometry. The ability to delivery siRNA was analyzed in HeLa cells. bLFcin(6) exhibited concentration-dependent uptake and intracellular distribution. Below 7.5 μm, uptake of bLFcin(6) was significantly lower than… CONTINUE READING