The site-specific deletion in plasmid pBR322.


The formation of a deletion derivative of plasmid pBR322, designated pBR322 delta 1, was observed during cloning of various eukaryotic DNAs, when the BamHI site of the plasmid vector was used for construction of the recombinant molecules. The restriction analysis of six independently isolated pBR322 delta 1 plasmids allowed establishment of their complete… (More)


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@article{Garaev1982TheSD, title={The site-specific deletion in plasmid pBR322.}, author={Mansur M. Garaev and Aleksei F Bobkov and Alyona Bobkova and Vjacheslav N. Kalinin and Valeri D Smirnov and Khudakov YuE and T. I. Tikchonenko}, journal={Gene}, year={1982}, volume={18 1}, pages={21-8} }