The simultaneous determination of oxidized and reduced glutathiones in liver tissue by ion pairing reverse phase high performance liquid chromatography with a coulometric electrochemical detector.


The oxidized (GSSG) and reduced (GSH) forms of glutathione were quantified simultaneously in rat liver tissues by ion-pairing reverse phase high pressure liquid chromatography coupled to a coulometric electrochemical detector (HPLC-EC). Other biological thiols namely cysteine, cystathionine, homocysteine and methionine were shown to be well resolved from… (More)


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