The seven amino acids of human RAMP2 (86) and RAMP3 (59) are critical for agonist binding to human adrenomedullin receptors.

@article{Kuwasako2001TheSA,
  title={The seven amino acids of human RAMP2 (86) and RAMP3 (59) are critical for agonist binding to human adrenomedullin receptors.},
  author={Kenji Kuwasako and Kazuo Kitamura and Kiyoharu Ito and Toshihiko Uemura and Yasuhiro Yanagita and Johji Kato and T. Sakata and Tanenao Eto},
  journal={The Journal of biological chemistry},
  year={2001},
  volume={276 52},
  pages={49459-65}
}
When co-expressed with a receptor activity-modifying protein (RAMP) accessory protein, calcitonin receptor-like receptor (CRLR) can function as a calcitonin gene-related peptide receptor (CRLR-RAMP1) or an adrenomedullin (AM) receptor (CRLR-RAMP2/3). Here we report on the structural domain(s) involved in selective AM binding that were examined using various RAMP chimeras and deletion mutants. Co-expression of chimeric RAMPs and CRLR in HEK293 cells revealed that residues 77-101, situated in the… CONTINUE READING