History of DNA Sequencing
- BiologyFolia Veterinaria
This review aims to highlight the evolution of DNA sequencing techniques and the machines used, including their principles and key achievements.
Generations of Sequencing Technologies: From First to Next Generation
This goal is to provide beginners in the field as well as to the amateurs of science a simple and understandable description of NGS technologies in order to provide them with basic knowledge as an initiation into this field in full ardor.
Metagenomic DNA Sequencing: Technological Advances and Applications
This chapter intends to provide information about past applied and current DNA sequencing approaches and will discuss their limitations and strength, as well as the application of these sequencing techniques.
High-Throughput Sequencing Technologies
- BiologyBioinformatics in Rice Research
This chapter presents a simple overview of the high-throughput sequencing technologies, their applications, and limitations to provide readers in the field with an easy and comprehensible description of HTS technologies to provide them with essential knowledge in full zeal.
Impact of Nucleic Acid Sequencing on Viroid Biology
- BiologyInternational journal of molecular sciences
The early 1970s marked two breakthroughs in the field of biology: (i) The development of nucleotide sequencing technology; and, (ii) the discovery of the viroids. The first DNA sequences were…
Whole-genome sequencing of eukaryotes: From sequencing of DNA fragments to a genome assembly
- Biology, EngineeringRussian Journal of Genetics
This review discusses the key technologies for the genome sequencing and the de novo assembly, as well as different approaches to improve the quality of existing drafts of genome sequences.
LUCS: a high-resolution nucleic acid sequencing tool for accurate long-read analysis of individual DNA molecules
A new technology termed LUCS (Long-molecule UMI-driven Consensus Sequencing), in which reads from third-generation sequencing are aggregated by unique molecular identifiers specific for each individual DNA molecule, which enables in-silico reconstruction of highly accurate consensus reads of each DNA molecule independent of other molecules in the sample.
Restriction enzymes and their use in molecular biology: An overview
- BiologyJournal of Biosciences
The history of restriction enzymes is celebrated in the light of their many different uses, as these proteins have accompanied the history of DNA for over 50 years representing active witnesses of major steps in the field.
A crash course in sequencing for a microbiologist
- BiologyJournal of Applied Genetics
This review will discuss applications of high-throughput methods to study bacteria in a much broader context than simply their genomes to lead to the reconstruction of regulatory networks within bacterial cells that allow communicating with the environment (signalome and interactome) and virtual cell reconstruction.
Nanopore Sequencing and its Application in Biology
- BiologyPostepy biochemii
Nanopore sequencing is a third generation sequencing technique. It involves the electrophoretic transport of nucleic acids through the protein channels of nanometer size, called nanopores, followed…
SHOWING 1-10 OF 123 REFERENCES
Single-Molecule DNA Sequencing of a Viral Genome
An amplification-free method for determining the nucleotide sequence of more than 280,000 individual DNA molecules simultaneously is reported, which demonstrates a strategy for high-throughput low-cost resequencing.
Next-generation DNA sequencing
- BiologyNature Biotechnology
Next-generation DNA sequencing has the potential to dramatically accelerate biological and biomedical research, by enabling the comprehensive analysis of genomes, transcriptomes and interactomes to become inexpensive, routine and widespread, rather than requiring significant production-scale efforts.
Large-scale and automated DNA sequence determination.
New sequencing methodologies, fully automated instrumentation, and improvements in sequencing-related computational resources may render genome-size sequencing projects (100 Mb or larger) feasible during the next 5 to 10 years.
New sequencing technologies
- BiologyClinical and Translational Oncology
The field is broadening applications that complement first, second and third generation sequencing systems to get high-resolution genetic information and fourth generation sequencing is on the horizon.
Sequence information can be obtained from single DNA molecules
- Biology, ChemistryProceedings of the National Academy of Sciences of the United States of America
These experiments show that one can study the activity of DNA polymerase at the single molecule level with single base resolution and a high degree of parallelization, thus providing the foundation for a practical single molecule sequencing technology.
DNA sequencing: bench to bedside and beyond†
- BiologyNucleic acids research
New ‘massively parallel’ sequencing methods are greatly increasing sequencing capacity, but further innovations are needed to achieve the ‘thousand dollar genome’ that many feel is prerequisite to personalized genomic medicine.
The complete genome of an individual by massively parallel DNA sequencing
This sequence was completed in two months at approximately one-hundredth of the cost of traditional capillary electrophoresis methods and demonstrated the acquisition of novel human sequence, including novel genes not previously identified by traditional genomic sequencing, which is the first genome sequenced by next-generation technologies.
Landscape of next-generation sequencing technologies.
- BiologyAnalytical chemistry
This Review concentrates on the technology behind the third- and fourth-generation sequencing methods: their challenges, current limitations, and tantalizing promise.
Accurate Whole Human Genome Sequencing using Reversible Terminator Chemistry
An approach that generates several billion bases of accurate nucleotide sequence per experiment at low cost is reported, effective for accurate, rapid and economical whole-genome re-sequencing and many other biomedical applications.
Molecular Structure of Nucleic Acids: A Structure for Deoxyribose Nucleic Acid
The determination in 1953 of the structure of deoxyribonucleic acid (DNA), with its two entwined helices and paired organic bases, was a tour de force in X-ray crystallography and opened the way for a deeper understanding of perhaps the most important biological process.