The sequence complementarity between HIV-1 5' splice site SD4 and U1 snRNA determines the steady-state level of an unstable env pre-mRNA.

@article{Kammler2001TheSC,
  title={The sequence complementarity between HIV-1 5' splice site SD4 and U1 snRNA determines the steady-state level of an unstable env pre-mRNA.},
  author={Susanne Kammler and Cordula Leurs and Marcel Freund and J{\"o}rg Krummheuer and Karsten Seidel and Thomas Ostergaard Tange and Mette Katrine Lund and J\orgen Kjems and A S Scheid and Heiner Schaal},
  journal={RNA},
  year={2001},
  volume={7 3},
  pages={421-34}
}
HIV-1 env expression from certain subgenomic vectors requires the viral regulatory protein Rev, its target sequence RRE, and a 5' splice site upstream of the env open reading frame. To determine the role of this splice site in the 5'-splice-site-dependent Rev-mediated env gene expression, we have subjected the HIV-1 5' splice site, SD4, to a mutational analysis and have analyzed the effect of those mutations on env expression. The results demonstrate that the overall strength of hydrogen… CONTINUE READING