The sensor kinase PhoQ mediates virulence in Pseudomonas aeruginosa.

@article{Gooderham2009TheSK,
  title={The sensor kinase PhoQ mediates virulence in Pseudomonas aeruginosa.},
  author={W. James Gooderham and Shaan Gellatly and François Sanschagrin and Joseph B. McPhee and Manjeet Bains and C{\'e}line Cosseau and Roger C. L{\'e}vesque and Robert E. W. Hancock},
  journal={Microbiology},
  year={2009},
  volume={155 Pt 3},
  pages={
          699-711
        }
}
Pseudomonas aeruginosa is a ubiquitous environmental Gram-negative bacterium that is also a major opportunistic human pathogen in nosocomial infections and cystic fibrosis chronic lung infections. PhoP-PhoQ is a two-component regulatory system that has been identified as essential for virulence and cationic antimicrobial peptide resistance in several other Gram-negative bacteria. This study demonstrated that mutation of phoQ caused reduced twitching motility, biofilm formation and rapid… 

Figures and Tables from this paper

The Pseudomonas aeruginosa PhoP-PhoQ Two-Component Regulatory System Is Induced upon Interaction with Epithelial Cells and Controls Cytotoxicity and Inflammation

Data indicate that mutation of phoQ results in a phenotype that is similar to the less virulent but more inflammatory phenotype of clinical strains isolated from chronic-stage cystic fibrosis lung infections.

The Sensor Kinase CbrA Is a Global Regulator That Modulates Metabolism, Virulence, and Antibiotic Resistance in Pseudomonas aeruginosa

Microarray analysis revealed that under swarming conditions, CbrA regulated the expression of many genes, including phoPQ, pmrAB, arnBCADTEF, dnaK, and pvdQ, consistent with the antibiotic resistance and swarming impairment phenotypes of the cbrA mutant.

A dynamic and intricate regulatory network determines Pseudomonas aeruginosa virulence

A ‘birds-eye’ view of the regulatory cascades provides the forum opportunities to pose questions, formulate hypotheses and evaluate theories in elucidating P. aeruginosa pathogenesis, and some regulators have been identified that modulate multiple virulence mechanisms.

Two-Component Signaling Systems Regulate Diverse Virulence-Associated Traits in Pseudomonas aeruginosa

Deletion strains for each of 64 genes encoding histidine kinases and one histidine phosphotransferase in P. aeruginosa PA14 are generated, providing new insight into the functions of two-component signaling proteins and providing a resource for future investigations.

Small molecules with antibiofilm, antivirulence and antibiotic synergy activities against Pseudomonas aeruginosa

An approach to identify P. aeruginosa biofilm inhibitors by repressing the production of the matrix exopolysaccharide (EPS) polymers by using a high throughput gene expression screen to identify compounds that repress expression of pel and psl genes.

Exopolysaccharide-Repressing Small Molecules with Antibiofilm and Antivirulence Activity against Pseudomonas aeruginosa

A high-throughput gene expression screen was used to identify compounds that repress expression of the pel genes and the combination of antibiotics and antibiofilm compounds increased killing of P. aeruginosa PAO1 virulence in the nematode slow killing model and represented a novel anti-infective strategy for the possible treatment of chronic P.aeruginose infections.

A PhoPQ-Regulated ABC Transporter System Exports Tetracycline in Pseudomonas aeruginosa

The results indicated that the PhoPQ-regulated ABC transporter is associated with intrinsic resistance to antibiotics and other adverse compounds in P. aeruginosa, probably by extruding them out of the cell.

Sensing Mg2+ contributes to the resistance of Pseudomonas aeruginosa to complement‐mediated opsonophagocytosis

It is demonstrated that expression of PhoQ is essential to detect Mg2+ and reduce the expression of OprH, a previously unrecognized C3 binding molecule that promotes the opsonophagocytosis of P. aeruginosa.

Two-component systems required for virulence in Pseudomonas aeruginosa

This minireview discusses the growing number of TCSs that have been implicated in the virulence of P. aeruginosa, with a special focus on the emerging theme of multikinase networks, which are networks comprising multiple sensor kinases working together, sensing and integrating multiple signals to decide upon the best response.

Evolutionary trade-offs associated with loss of PmrB function in host-adapted Pseudomonas aeruginosa

Pseudomonas aeruginosa pmrB mutations in CF patients are proposed to be subject to an evolutionary trade-off, leading to enhanced colonisation potential, CFTR inhibition, and resistance to host defences, but also to increased susceptibility to antibiotics.
...

References

SHOWING 1-10 OF 58 REFERENCES

Regulation of virulence and antibiotic resistance by two-component regulatory systems in Pseudomonas aeruginosa.

The current body of knowledge of these and other two-component systems that provides insight into the complex regulation of virulence and resistance in P. aeruginosa are summarized.

The Transcriptional Regulator AlgR Is Essential for Pseudomonas aeruginosa Pathogenesis

It is found that P. aeruginosa PAO700 (algR::Gmr) was less lethal than PAO1, as tested in an acute septicemia infection mouse model, and was cleared more efficiently in a mouse pneumonia model, suggesting that AlgR plays both a positive role and a negative role in gene expression.

Role of Pseudomonas aeruginosa PhoP-phoQ in resistance to antimicrobial cationic peptides and aminoglycosides.

Significant evidence is provided that OprH is not involved in P. aeruginosa aminoglycoside resistance and that the changes in resistance in strain H855 and a previously reported oprH mutant were due to polar effects on phoP-phoQ rather than loss of OPRH expression.

Identification of Pseudomonas aeruginosa Genes Involved in Virulence and Anaerobic Growth

Novel genes important for anaerobic growth are identified and it is demonstrated that an aerobic metabolism influences virulence of P. aeruginosa.

Pseudomonas aeruginosa Acquires Biofilm-Like Properties within Airway Epithelial Cells

Findings suggest that P. aeruginosa can use airway epithelial cells as a sanctuary for persistence and develop a reversible antibiotic resistance phenotype characteristic of biofilm physiology that can contribute to development of chronic infection.

Microarray Analysis of Global Gene Expression in Mucoid Pseudomonas aeruginosa

The recognition of genes induced in mucoid P. aeruginosa, other than those associated with alginate biosynthesis, reported here revealed the identity of previously unappreciated factors potentially contributing to the morbidity and mortality caused by mucoidy.

The Salmonellae PhoQ sensor: mechanisms of detection of phagosome signals

Pseudomonas aeruginosa and Salmonella typhimurium PhoQ are compared, providing evidence that a subset of Pho Q proteins of environmental organisms evolved more limited sensing capability using a different structural sensing mechanism.

Pathogen-host interactions in Pseudomonas aeruginosa pneumonia.

In addition to antibiotic therapy, strategies directed toward enhancing host defense and/or limiting excessive inflammation could be important to improve outcome in P. aeruginosa lung infections.

PhoP–PhoQ homologues in Pseudomonas aeruginosa regulate expression of the outer‐membrane protein OprH and polymyxin B resistance

Measurement of the catechol‐2,3‐dioxygenase activity, expressed from the xylE transcriptional fusion in strains H851 and H854, indicated that PhoP–PhoQ is involved in the regulation of phoP-phoQ as well as oprH.
...